Applications of Chromatography

 Chromatography is regarded as a powerful separation tool employed in all branches of sciences and it is usually the only methods of separating components out of complex mixtures. A great variety of chromatographic procedures deals with differences in charge, binding affinities, size and many other properties. Different kinds of chromatography have been developed so far. They include column chromatography, gas chromatography, high performance liquid chromatography, ion exchange chromatography and size exclusion chromatography, etc. The different types have been utilized for different applications. Out of those types, the application of high performance liquid chromatography in the purification and separation of proteins and peptides is highly popular. Whatever is the complexity or intricacy of the scale of purification process, the Preparative Chromatography systems are developed to meet the changing needs. Along with the broad variety of high performance flash chromatography columns, optimized solution is being offered to suit the purification workflow.

The Preparative Analytical Chromatography systems have been implemented to improve the speed and purity of separation. It is possible to perform high speed fabrication by means of a column with high separation capacity. Chromatography is typically the process involving fractionation of mixtures by carrying out constant partition between two phases, the one of which will be moving ahead the other. In principle, there seems to be no constraint on the nature of stationary and mobile phases or on the kinds of equilibria engaged in the partition. In real time, the most essential applications involved a solid or liquid stationary phase and a liquid mobile phase.

Chromatography has been utilized extensively in characterization, determination and isolation of the carotenoids and remains to be the method of choice in examining the new materials for carotenoids. The examination of faecal porphyrins, urinary and bile pigments by chromatographic methods are presently the diagnostic aids. The Application of Preparative Chromatography to peptides, proteins and amino acids has formed the basis of several reviews. The latest developments in study on this group of compounds has allowed the extensions of the quantitative and qualitative methods for amino acids, the examination of proteins, peptides and enzymes, liver fractions, insulin and urinary excretion of amino acids and nitrogen metabolism of thyroids. 

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